PERK(proteinkinaseR-likeendoplasmicreticulumkinase)isatypeImembraneproteinlocatedintheendoplasmicreticulum(ER).ERstressincreasestheactivityofPERK,whichthenphosphorylateseIF2αtopromotereducedtranslation,leADIngtoitsinactivation,andthustoarapidreductionoftranslationalinitiationandrepressionofglobalproteinsynthesis.PERK-deficientmicehavedefectsinpancreaticβcellsseveralweeksafterbirth,suggestingaroleforPERK-mediatedtranslationalcontrolinprotectingsecretorycellsfromERstress.PERKactivationduringERstresscorrelateswithautophosphorylationofitscytoplasmickinasedomain.PhosphorylationofPERKatSer713canbeusedasaMarkerforitsactivationstatus.ThepredictedphosphorylationsiteandsurroundingresiduesofSer713are:HIEIIAPS*PQRSRSF.

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