Macrophages are produced by differentiation of monocyte in response to an infection or tissue damage. Their primary function is to recognize, engulf, and destroy target cells including pathogens, dying or dead cells, and cellular debris. Like dendritic cells, macrophages are also professional antigen presenting cells that play a crucial role in initiating an immune response. Macrophages secrete an array of cytokines which aid in host defense, tissue repair, and immunoregulation. Macrophages can be divided into multiple subtypes based on different functions. Inflammation-encouraging M1 macrophages produce proinflammatory cytokines, such as CXCL1 (KC), IL-18, IL-23, IL-12p70, IL-6, TNF-α, IL-12p40, and IL-1β. Anti-inflammatory and tissue-repairing M2 macrophages decrease immune reactions and promote tissue repair by releasing a different set of factors, such as Free Active TGF-β1, CCL22 (MDC), IL-10, IL-6, G-CSF, and CCL17 (TARC).Due to their important roles in immune responses, macrophages are critically involved in a variety of inflammatory disorders, such as sepsis-related multiple organ dysfunction/failure, microbial infection, acute injuries, neurodegenerative disorders, cancer, cardiovascular, and autoimmune diseases. Measuring the mediators of proinflammation and anti-inflammation produced by macrophages may help not only in understanding the fundamental functions of macrophages, but also in finding the mechanisms of various pathological processes.The LEGENDplex^TM Mouse Macrophage/Microglia Panel (13-plex) is a bead-based multiplex assay panel, using fluorescence-encoded beads suitable for use on various flow cytometers. It allows for simultaneous quantification of 13 key targets involved in monocyte differentiation and macrophage functions such as CXCL1 (KC), Free Active TGF-β1, IL-18, IL-23, CCL22 (MDC), IL-10, IL-12p70, IL-6, TNF-α, G-CSF, CCL17 (TARC), IL-12p40, and IL-1β. This assay panel provides higher detection sensitivity and broader dynamic range than traditional ELISA methods. The panel has been validated for use on cell culture supernatant and serum.The LEGENDplex™ Mouse Macrophage/Microglia Panel (13-plex) is designed to allow flexible customization within the panel. It can also be divided into subpanels such as:

LEGENDplex™ Mouse M1 Macrophage Panel (8-plex)LEGENDplex™ Mouse M2 Macrophage Panel (6-plex)Please visit www.biolegend.com/legendplex for more information on how to mix and match within the panel.This assay is for research use only.

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