| 货号： | 502204 |
| 克隆性： | 否 |
| 保存条件： | 蓝冰 |
| 规格： | 500 ug |
| Clone | BVD2-23B6 |
| Isotype | Rat IgG2a, κ |
| Reactivity | Human |
| Immunogen | E. coli -expressed, recombinant human GM-CSF |
| Formulation | 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test. |
| Preparation | The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography. |
| Concentration | 1mg/ml |
| Recommended Usage | Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.25-1 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of GM-CSF recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application. |
| Applications | ELISA Capture, ELISPOT Capture, Neut, IP, WB |
| Application Notes | ELISA or ELISPOT Capture1-6: The Purified BVD2-23B6 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD2-21C11 antibody (Cat. No. 502304) as the detecting antibody. The LEAF™ Purified antibody is suggested for ELISPOT capture. Neutralization1-4: The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human GM-CSF bioactivity (Cat. No. 502204). Additional reported applications (for the relevant formats) include: immunoprecipitation3 and Western blotting. Note: For testing human GM-CSF in serum or plasma, BioLegends ELISA Max™ Sets (Cat. No. 432001 to 432006) are specially developed and recommended. |
| Application References | 1. Bacchetta R, et al. 1990. J. Immunol. 144:902. 2. Kita H, et al. 1991. J. Exp. Med. 174:745. 3. Abrams J, et al. 1992. Immunol. Rev. 127:5. 4. Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133. 5. Mahanty S, et al. 1992. J. Immunol. 148:3567. 6. Klinman D, et al. 1994. Curr. Prot. Immunol.. John Wiley and Sons New York. Unit 6.19. |
| Description | Granulocyte/macrophage - colony stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T cells, B cells, mast cells, macrophages, fibroblasts and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte and eosinophil progenitors. The BVD2-23B6 antibody reacts with human granulocyte/macrophage - colony stimulating factor (GM-CSF). The BVD2-23B6 antibody can neutralize the bioactivity of natural or recombinant GM-CSF. |
| Storage | The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions. |
